Part:BBa_I718002:Design

Diacylglycerol-Acyl-transferase (DGAT) for triglyceride synthesis

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 147
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We mutagenized Pst-1 site in position 1062 after ATG. We mutagenized stop codon TAAAAA into TAATAA to conform with BioBrick standards. We added in 5' a strong RBS (BBa_B0030) and prefixe biobrick (BBa_G00000). In 3', we added suffixe biobrick (BBa_G00001).

Source

• Acinetobacter calcoaceticus ADP1
• Accession number: AF529086
• Kalscheuer,R. and Steinbuchel,A. A Novel Bifunctional Wax Ester Synthase/Acyl-CoA:Diacylglycerol Acyltransferase Mediates Wax Ester and Triacylglycerol Biosynthesis in Acinetobacter calcoaceticus ADP1. J. Biol. Chem. 278 (10), 8075-8082 (2003)

References

Kalscheuer,R. and Steinbuchel,A. A Novel Bifunctional Wax Ester Synthase/Acyl-CoA:Diacylglycerol Acyltransferase Mediates Wax Ester and Triacylglycerol Biosynthesis in Acinetobacter calcoaceticus ADP1. J. Biol. Chem. 278 (10), 8075-8082 (2003)

Stoveken T, Kalscheuer R, Malkus U, Reichelt R, Steinbuchel A. The wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1: characterization of a novel type of acyltransferase. J Bacteriol. ;187(4):1369-76 (2005)